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standard epifluorescence microscope with a chilled ccd camera  (Hamamatsu)

 
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    Structured Review

    Hamamatsu standard epifluorescence microscope with a chilled ccd camera
    Standard Epifluorescence Microscope With A Chilled Ccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/standard+epifluorescence+microscope+with+a+chilled+ccd+camera/standard+epifluorescence+microscope+with+a+chilled+ccd+camera/pmc04166168-69-29-41
    Average 90 stars, based on 1 article reviews
    standard epifluorescence microscope with a chilled ccd camera - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microscopy:

    Article Title: Loss of Glial Neurofascin155 Delays Developmental Synapse Elimination at the Neuromuscular Junction
    Article Snippet: .. Muscle, nerve, and spinal cord preparations were viewed using a phase-contrast microscope with a chilled CCD camera (40× objective, for muscle fiber measurements), a standard epifluorescence microscope with a chilled CCD camera (20× and 40× objective, 0.8 NA, Nikon IX71 microscope, Hammamatsu C4742-95; for endplate area, endplate number, and endplate maturation), an upright fluorescence microscope (40× and 60× objective, for polyinnervation and axonal input per NMJ), or a laser scanning confocal microscope (40× and 60× objective, 1.4 NA, Zeiss LSM710; number of axons innervating a muscle, polyinnervation counts, preterminal axon and axon terminal fluorescence intensity, spinal cord ventral root fluorescence intensity, peripheral nerve fluorescence intensity, and axonal input per NMJ). .. Whole muscles/sections were incubated in 2% Triton X in 0.1 m PBS for 30 min, blocked in a solution of 4% BSA and 1% Triton X in 0.1 m PBS for 30 min before overnight incubation with primary antibodies raised against 200 kDa neurofilament/neurofilament-heavy (NF-H) (rabbit, 1:1000, Abcam), 165 kDa neurofilament/neurofilament-medium (NF-M) (mouse, 1:200, Developmental Studies Hybridoma Bank), 70 kDa neurofilament/NF-L (rabbit, 1:1000, Millipore), and β3-tubulin (rabbit, 1:1000, Abcam) in blocking solution.

    Fluorescence:

    Article Title: Loss of Glial Neurofascin155 Delays Developmental Synapse Elimination at the Neuromuscular Junction
    Article Snippet: .. Muscle, nerve, and spinal cord preparations were viewed using a phase-contrast microscope with a chilled CCD camera (40× objective, for muscle fiber measurements), a standard epifluorescence microscope with a chilled CCD camera (20× and 40× objective, 0.8 NA, Nikon IX71 microscope, Hammamatsu C4742-95; for endplate area, endplate number, and endplate maturation), an upright fluorescence microscope (40× and 60× objective, for polyinnervation and axonal input per NMJ), or a laser scanning confocal microscope (40× and 60× objective, 1.4 NA, Zeiss LSM710; number of axons innervating a muscle, polyinnervation counts, preterminal axon and axon terminal fluorescence intensity, spinal cord ventral root fluorescence intensity, peripheral nerve fluorescence intensity, and axonal input per NMJ). .. Whole muscles/sections were incubated in 2% Triton X in 0.1 m PBS for 30 min, blocked in a solution of 4% BSA and 1% Triton X in 0.1 m PBS for 30 min before overnight incubation with primary antibodies raised against 200 kDa neurofilament/neurofilament-heavy (NF-H) (rabbit, 1:1000, Abcam), 165 kDa neurofilament/neurofilament-medium (NF-M) (mouse, 1:200, Developmental Studies Hybridoma Bank), 70 kDa neurofilament/NF-L (rabbit, 1:1000, Millipore), and β3-tubulin (rabbit, 1:1000, Abcam) in blocking solution.



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